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What is ELISA?
The enzyme-linked immunosorbent assay (ELISA for short) is a technique used to detect the presence of an antibody or an antigen in a sample.
It utilizes two antibodies, one of which is specific to the antigen and another that is coupled to an enzyme. This second antibody gives the assay its enzyme-linked name, and will cause a chromogenic substrate to produce color wherever it is bound.
ELISA is a powerful antibody-based biodetection tool used in the field to hunt for pathogens in water, food, or air, whether they emerge naturally or through acts of aggression. (Source: Bio-Rad Laboratories)
Download a flow chart of the ELISA process (PDF document by Bio-Rad Laboratories).
Consult the Bio-Rad Laboratories' ELISA Lab Kit Instructions, "ELISA Immuno Explorer Kit," for more information for Teaching Assistants and High Schools who are also interested (Web Page).